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相关概念视频

Leaky Scanning02:28

Leaky Scanning

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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相关实验视频

Updated: May 17, 2025

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

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解决基于测序的HIV-1表转录组学

Michael S Bosmeny1, Joao I Mamede2, Keith T Gagnon1

  • 1Department of Biochemistry, Wake Forest University, School of Medicine, Winston-Salem, NC, USA.

Epigenomics
|May 16, 2025
PubMed
概括
此摘要是机器生成的。

了解HIV-1RNA的化学修饰 (HIV-1表谱学) 是至关重要的,但具有挑战性. 纳米孔直接RNA测序为更好的HIV-1表转录组分辨率提供了一种标准化的方法.

关键词:
艾滋病病毒-1 艾滋病病毒-1基因组RNA的修饰是RNA的修饰.标志性转录组学 标志性转录组学这是一个纳米孔.测序的测序是指测序的测序.

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Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
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相关实验视频

Last Updated: May 17, 2025

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Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
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科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 艾滋病毒-1表转录学,研究艾滋病毒-1的RNA化学修饰,尚未完全理解.
  • 不同的方法和数据使得对HIV-1表转录组的统一观点变得复杂.
  • 关键的修改包括N6 - 甲基亚丁素 (m6A),5-甲基细胞素 (m5C),伪尿素 (Ψ),2'-O-甲基化 (Nm) 和N4-乙基素 (ac4C).

研究的目的:

  • 审查目前用于识别和解释HIV-1RNA修饰的方法.
  • 为了突出解决HIV-1表写体的挑战.
  • 为未来的研究推一个标准化的测序策略.

主要方法:

  • 基于抗体的选择方法的审查.
  • 基于化学处理的选择方法的审查.
  • 评估纳米孔直接RNA测序用于修饰检测.

主要成果:

  • 现有的HIV-1表转录组分析方法差异很大.
  • 纳米孔直接RNA测序显示了准确的修饰检测的前景.
  • 为了进行跨研究比较,需要进行标准化.

结论:

  • 对HIV-1表写体的共识观点需要标准化的方法.
  • 建议纳米孔直接RNA测序作为标准化策略.
  • 采用这种方法将改善基准测试并解决HIV-1表谱学问题.