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用si-circETS1装载的PLGA微球作为一种治疗策略,以延迟椎间盘退化

Wenlei Nie¹, Rong Zhang¹, Pingfeng Xie¹

¹Department of Orthopaedics, The First People's Hospital of Wuhu City, No. 1 Chizhushan East Road, Wuhu, 241000 Anhui China.

|May 16, 2025

概括

这项研究确定circETS1是椎间盘退化 (IDD) 的关键因素. 通过PLGA微球输送si-circETS1,通过减少炎症和促进组织修复,有效治疗IDD.

科学领域:

分子生物学分子生物学
生物材料科学生物材料科学
再生医学是一种再生医学.

背景情况:

椎间盘退化 (IDD) 导致严重的慢性腰部疼痛和功能障碍.
循环RNAs (circRNAs) 正在成为细胞功能的关键调节者和IDD的潜在治疗点.

研究的目的:

研究circETS1在细胞核中的作用,并开发IDD的新疗法.
评估用于IDD治疗的充满si-circETS1的聚乳-co-glycolic acid (PLGA) 微球的疗效.

主要方法:

循环RNA测序 (circRNA-seq) 在退化的磁盘组织中发现了升高的circETS1.
在人体初级NPCs的体外研究评估了circETS1对增殖,细胞亡和细胞外基质 (ECM) 代谢的影响,使用RT-qPCR,西方涂抹,流细胞计和免疫光.
在体外和体内实验中,评估了PLGA微球提供si-circETS1.

主要成果:

在退化的核肺组织中,CircETS1的表达显著上调.
通过PLGA微球传递si-circETS1有效下调了NPC中的circETS1表达.
治疗减少了炎症,增强了ECM合成和修复,并在体外和体内推迟了IDD进展.

结论:

CircETS1在IDD的发病过程中起着至关重要的作用.
通过PLGA微球介导的si-circETS1输送是IDD的一种有前途的治疗策略,具有临床应用的潜力.

关键词:

循环1 循环1 椎间盘退化椎间盘退化微球是微球的组成部分.细胞的核是脉细胞.聚乳-同-甘氨酸酸的多样性.

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