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Updated: May 21, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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Published on: November 1, 2024

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快速的两步目标捕获确保了有效的CRISPR-Cas9引导的基因组编辑

Honglue Shi, Noor Al-Sayyad, Kevin M Wasko

    bioRxiv : the preprint server for biology
    |May 16, 2025
    PubMed
    概括

    克里斯普尔-Cas9基因组编辑效率随着原体空间邻基因特异性 (PAM) 的降低而降低. 广泛的PAM识别导致非选择性的DNA结合和细胞中较低的编辑成功.

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