相关实验视频

Updated: Aug 8, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

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开发了一种基于EvaGreen染料的新型重组酶辅助放大试验,使用自我避开的分子识别系统原料

Xinxin Shen1, Shaowei Hua1, Zijin Zhao1

  • 1National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

China CDC weekly
|May 16, 2025
PubMed
概括

一种新的基于EvaGreen染料的测定方法简化了牛奶中的病原体检测. 与传统方法相比,这种新的方法为识别Pseudomonas fluorescens和Bacillus cereus等细菌提供了更高的灵敏度.

关键词:
细菌花类植物 (Bacillus cereus) 是一种Eva绿色染料 绿色染料是一种染料.融化曲线的曲线伪虫光体 伪虫光体生牛奶 生牛奶是什么意思再组合酶辅助的放大.萨姆斯海峡 (SAMSRS) 是一个

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction03:38

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...