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与三胺和RSL3同时治疗会诱导肝细胞癌细胞亡和铁亡

Weixia Liu¹, Guodi Wu¹, Jing Wang¹

¹The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Afﬁliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China.

Molecular medicine reports

|May 16, 2025

概括

肝细胞癌 (HCC) 细胞用三胺 (TPL) 和RSL3,一种GPx4抑制剂治疗,显示细胞死亡增加. 这种组合疗法诱导了亡和铁亡,增强了抗癌疗效.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
在瘤学瘤学.

背景情况:

谷氨酸过氧化酶4 (GPx4) 通过减少脂质过氧化,对抑制细胞死亡途径如细胞亡和铁亡至关重要.
在三胺 (TPL) 治疗后,肝细胞癌 (HCC) 细胞中的GPx4蛋白水平升高表明可能对TPL诱导的细胞毒性产生抗性.
抑制GPx4活性是一种增强抗癌疗法的潜在策略.

研究的目的:

研究TPL和RSL3 (一种GPx4抑制剂) 对HCC细胞的联合作用.
通过准GPx4.4,确定联合治疗是否可以克服TPL耐药性.
探索诱导HCC细胞中的亡和铁亡.

主要方法:

细胞计数工具-8测试细胞活力.
流细胞测量用于亡分析.
西方斑点测试测量GPx4蛋白质水平.

主要成果:

同时使用TPL和RSL3显著促进HCC细胞亡.
在组合治疗后,细胞内活性氧物种 (ROS) 水平升高.
结合疗法有效诱导HCC细胞中的铁亡.

结论:

与TPL和RSL3同时治疗可以增强HCC细胞的抗增殖功效.
这种组合疗法有效地诱导了细胞死亡途径的apoptotic和ferroptotic.
针对GPx4活动与TPL治疗一起,为HCC治疗提供了一个有希望的策略.

关键词:

RAS-选择性致命的 3 选择性致命的灭症 (apoptosis) 是一种死亡的过程.铁性化 (ferroptosis) 是一种谷氨过氧化酶4的作用肝细胞癌是肝细胞癌.有活性氧物种的反应性氧物种.三托利化物是什么

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