时间依赖的EDTA对白细胞分数和形态的影响,与直接指纹刺伤所产生的血涂抹相比
Anna Irding1, Filip Landgren1, Elisabeth Aardal2
1Department of Clinical Chemistry, Linköping University, Linköping, Sweden.
International journal of laboratory hematology
|May 16, 2025
概括
从乙烯基二胺三酸 (EDTA) 血液样本中,白血球差异计数可靠长达12小时. 然而,准确的形态评估需要在8小时内准备涂抹以避免变化.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 实验室医学 实验室医学
背景情况:
- 乙二胺酸 (EDTA) 通常用于血液样本的抗凝剂.
- 目前的建议建议在4小时内从EDTA中准备血涂片,因为可能发生白细胞变化.
- 有限的数据存在于形态评估的时间限制,特别是比较K2EDTA与直接指尖刺伤方法.
研究的目的:
- 确定从EDTA准备血涂抹的最佳时间框架,以准确评估白细胞形态.
- 通过时间比较EDTA样本的白细胞计数和形态的可靠性与指针刺伤样本的可靠性.
主要方法:
- 使用了37名患有白细胞数异常的患者的血液样本.
- 从EDTA管中以每4小时的间隔准备涂抹,直到采样后24小时.
- 对照涂抹是从直接的指尖刺伤中制成的.
- 涂抹被评估为差异细胞数量,形态变化和整体涂抹质量.
主要成果:
- 与指针刺伤对照组相比,白血球差异计数在采样后12小时内保持一致.
- 在血液采集后8小时以上准备的涂抹中观察到显著的形态变化.
- 在12小时内,中性粒细胞,淋巴细胞,单细胞,氨基细胞,基细胞和爆细胞的细胞数量没有显著差异.
结论:
- 手动分白细胞计数可以在收集后12小时内可靠地在EDTA血涂片上进行.
- 为了对白细胞进行全面的形态评估,需要在EDTA血液采样后8小时内准备涂抹.
更多相关视频
12:29Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
Published on: April 19, 2017
10.1K
14:45Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Published on: September 16, 2012
14.9K
相关概念视频
Effects of EDTA on End-Point Detection Methods
226
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
226
EDTA: Direct, Back-, and Displacement Titration
2.2K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
2.2K
EDTA: Indirect and Alkalimetric Titration
743
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
743
Masking and Demasking Agents
2.2K
EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
2.2K
