光和多酸盐激酶2协同调节深海细菌中零价值硫的产生
Tianhang Zhang1,2,3,4, Ruining Cai1,2,4, Chaomin Sun1,2,3,4
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology & Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
mSystems
|May 16, 2025
概括
深海细菌Erythrobacter flavus 21-3使用细菌菌素BPHP-15570感知红外光,激活零价值硫 (ZVS) 生产. 聚酸激酶2 (PPK2) 作为负调节剂,防止有毒ZVS的积累.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 深海生态 深海生态
背景情况:
- 深海微生物拥有专门的光感应机制.
- 之前的研究表明,蓝光促进了Erythrobacter flavus 21-3.的零价值硫 (ZVS) 生产.
- 长波长光在深海环境中更为普遍.
研究的目的:
- 研究E. flavus 21-3.中的红外光传感机制.
- 确定红外光如何调节ZVS生产.
- 确定参与ZVS生物合成及其控制的调节途径.
主要方法:
- 细菌分离和培养来自深海冷的细菌.
- 确定参与光感应和ZVS生产的关键蛋白质 (BPHP-15570,DGC-0450,mPilZ-1753,PPK2).
- 对调节c-di-GMP生物合成和ZVS生成的信号通路的分析.
主要成果:
- 细菌基基血BPHP-15570感应940nm的红外光.
- 红外光诱导BPHP-15570的自酸化,激活DGC-0450进行c-di-GMP合成.
- PPK2与GTP竞争,降低c-di-GMP生物合成和ZVS生产的调节,以防止毒性.
结论:
- E. flavus 21-3 使用复杂的途径来感知蓝光和红外光,用于ZVS生产.
- PPK2作为一个关键的负调节剂,保持安全的细胞内ZVS水平.
- 这项研究为深海环境中的光利用和硫循环提供了一个模型.
相关概念视频
01:27Cell Inclusions
1.4K
Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...
1.4K
01:30Anoxygenic Photosynthesis
1.9K
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
1.9K
01:20Sulfur Assimilation
554
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
554
01:17Gene Regulation During Sporulation
713
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
713
01:28Anoxygenic Phototrophic Bacteria
1.3K
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
1.3K
01:29Microbes and the Sulfur Cycle
123
Sulfur is a vital element in Earth's biogeochemical systems. It transitions through various inorganic states, including sulfate (SO₄²⁻), elemental sulfur (S⁰), and sulfide (S²⁻). Abiotic and biological mechanisms across oxic and anoxic environments intricately mediate these transformations. Sulfate, the most oxidized form of sulfur, is predominantly stored in rocks, marine sediments, and oceanic waters, acting as a long-term reservoir in the global sulfur...
123