主体PIK3C3促进Shigella flexneri通过真空球形成从细胞传播到细胞
Steven J Rolland1, Zachary J Lifschin1, Erin A Weddle1
1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, United States of America.
PLoS pathogens
|May 16, 2025
概括
PIK3C3对于Shigella flexneri的细胞间传播至关重要,使空洞状突起能够成熟成为双膜空洞. 抑制PIK3C3可以减少感染在细胞和幼的传播.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 传染性疾病 传染性疾病
背景情况:
- 石格拉柔性菌通过入侵结肠上皮细胞并在它们之间传播,导致细菌性痢疾.
- 这种细胞对细胞的传播涉及基于actin的运动性,形成膜突起,这些突起分解为真空状突起 (VLPs) 和最终的双膜真空 (DMVs).
研究的目的:
- 为了确定Shigella flexneri细胞间传播至关重要的宿主因素.
- 阐明PIK3C3在真空状突起到双膜真空过渡中的作用.
主要方法:
- 使用HT-29细胞和一个婴儿子模型的石病.
- 采用活光共聚焦显微镜来追踪细菌传播和真空球动态.
- 研究了PIK3C3抑制对感染焦点大小和细菌传播的作用.
主要成果:
- 确定PIK3C3对于Shigella flexneri细胞间传播至关重要,在抑制后减少感染焦点大小.
- 需要PIK3C3才能有效地将VLP分解为DMV,包括PtdIns(3) P积累和Dynamin 2招募.
- 抑制PIK3C3也影响了Listeria monocytogenes的传播,特别是在真空细胞逃逸阶段.
结论:
- 在Shigella flexneri传播中,PIK3C3发挥着关键作用,通过PtdIns(3) P和Dynamin 2.P,促进VLP通过PtdIns(3) P和Dynamin 2.P成长为DMV.
- 抑制PIK3C3有效地减少了Shigella flexneri在细胞和动物模型中的传播.
相关概念视频
01:26Bacterial Translocation and Protein Secretion
256
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
256
01:27Diversity of Protists IV
565
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
565
01:10Intracellular Movement of Viruses and Bacteria
3.2K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.2K