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Updated: Jul 2, 2026

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Metagenomic Analysis of Silage
Published on: January 13, 2017
在用于植物性牛奶替代品的植物原料中形成子的细菌的流行率
Anne Gleissle1, Herbert Schmidt2, Jörg Hinrichs1
1Department of Soft Matter Science and Dairy Technology, Germany.
International journal of food microbiology
|May 16, 2025
概括
对植物性牛奶原料的微生物分析显示了大量的子污染,影响了热处理要求. 了解细菌负载对于确保植物性饮料的安全性和保质期至关重要.
科学领域:
- 食品微生物学 食品微生物学
- 食品中的微生物安全性
- 植物性食品加工 植物性食品加工
背景情况:
- 植物性牛奶替代品 (PBMA) 越来越受欢迎,但它们的微生物安全性取决于原材料质量.
- 麦,杏仁,豆类和大米等原材料中的微生物负载有很大的变化,影响加工参数.
- 原材料中的高子数量对实现PBMA商业无菌性构成挑战.
研究的目的:
- 量化各种植物原料中的微生物负载,包括可活细胞和子.
- 为了确定这些原材料中占主导地位的细菌物种.
- 建立计算PBMAs必要热处理参数的基础.
主要方法:
- 对面粉,片,蛋白质分离物和麦,杏仁,豆和大米的糖进行微生物学测试.
- 分析包括中性活细胞计数 (mVCC),热性活细胞计数 (tVCC),中性子计数 (mSC) 和热性子计数 (tSC).
- 使用16S rRNA基因测序来识别细菌物种.
主要成果:
- 植物原料的微生物负载在1~8.5 log10 CFU/g之间,其中子比例很高.
- 麦和大米糖,即使经过UHT处理,也含有1到4个log10 CFU/g的子.
- 在435个分离物中,包括B. licheniformis,B. subtilis和B. tequilensis在内的细菌物种是最普遍的.
结论:
- 植物原料中的初始子负载是决定所需的热处理效率 (B*) 的关键因素.
- 显著的微生物污染需要强大的加工,以确保商业无菌的PBMAs.
- 精确评估微生物负载对于优化UHT处理和保证产品安全至关重要.
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