相关实验视频

Updated: Apr 1, 2026

Author Spotlight: Designing Simple and Inexpensive Techniques to Grow Methane-Oxidizing Bacteria in the Laboratory

Author Spotlight: Designing Simple and Inexpensive Techniques to Grow Methane-Oxidizing Bacteria in the Laboratory

Published on: September 6, 2024

在培养肉类中基因组规模的代谢模型:进步,挑战和未来的方向

Sandra Gomez Romero¹, Isabella Spielmann², Nanette Boyle³

¹Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, CO, USA.

Current opinion in biotechnology

|May 16, 2025

概括

基因组规模的代谢模型 (GEMs) 可以通过解决代谢挑战并实现无血清介质来优化培养肉类生产. 这种方法增强生物质生产,为传统肉类提供可持续和可扩展的替代品.

科学领域:

生物技术是生物技术.
细胞农业细胞农业
代谢工程是代谢工程.

背景情况:

培养肉类为传统肉类提供了可持续的替代品,但面临着诸如无血清介质要求和生长条件优化等代谢挑战.
基因组规模代谢模型 (GEMs) 是优化生物制造中的细胞代谢的强大工具,已成功应用于中国仓鼠卵巢细胞.
适应培养肉类的GEM可以解决关键的代谢障碍,提高生产效率.

研究的目的:

探索基因组规模代谢模型 (GEMs) 在克服培养肉类生产中的代谢挑战中的应用.
展示GEM如何为培养肉类提供成本效益高,无血清介质的转型.
突出GEM在提高生物质生产和培养肉的整体可扩展性方面的潜力.

主要方法:

使用基因组规模代谢模型 (GEMs) 衍生出的代谢流量分析.
从生物制造 (例如,中国仓鼠卵巢细胞) 调整已建立的GEM,用于培养肉类应用.
将多omics数据 (转录组,蛋白组) 与GEM集成,以提高预测准确度.

主要成果:

在肉类培养系统中,GEM可以确定细胞生长和生物质生产的最佳代谢条件.
应用GEM有助于开发具有成本效益的无血清培养基.
由GEMs指导的代谢工程策略可以显著提高养殖肉类生产的效率.

结论:

基因组规模的代谢模型 (GEMs) 对于克服培养肉类生产中的代谢限制至关重要.
利用GEM可以开发可扩展,可持续和经济可行的养殖肉类.
未来的研究应该集中在扩大代谢数据集和整合多omics数据,以完善培养肉的GEM.

更多相关视频

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

相关概念视频

Scale-Up Processes

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Metabolism of Chemolithotrophs

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.

Operon Model

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...

Overview of Metabolism

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

Metabolic Rate

Metabolic Rate

The human body is a powerhouse of energy, with every cell performing numerous functions that require energy. This energy production and consumption is measured by the metabolic rate, which quantifies the total heat generated by all the body's chemical reactions and mechanical work. This measurement helps to determine the rate of kilocalorie (kcal) consumption needed to fuel all ongoing activities.
The Basal Metabolic Rate (BMR) measures the energy expended at rest.
Several factors influence...