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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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相关实验视频

Updated: Jun 19, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

在体内通过全基因组方法对规范元素进行分析.

Kami Ahmad1, Steven Henikoff2

  • 1Basic Science Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.

Current opinion in structural biology
|May 16, 2025
PubMed
概括

细胞中的基因调节是复杂的. 新技术揭示了核中的动态色素运动是如何对基因表达至关重要的,进步了我们对因子-DNA相互作用的理解.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞基因调节在生物化学层面得到了很好的研究,重点是因子-DNA结合和蛋白质-DNA相互作用.
  • 在体外研究定义了分子相互作用的基本原理,但体外核环境呈现出独特的复杂性.

研究的目的:

  • 探索动态核过程在基因调节中的作用.
  • 要突出技术的进步观察体内因子-DNA相互作用.

主要方法:

  • 利用尖端技术在体内观察因子-DNA相互作用.
  • 采用高分辨率的染色体分析方法来研究核动力学.

主要成果:

  • 在体内研究显示,细胞核是基因调节至关重要的动态区.
  • 由分子电机驱动的染色体动力学显著影响基因表达.

结论:

  • 了解体内染色质动态对于了解真核生物基因调节的完整画面至关重要.
  • 先进的染色体分析方法是揭示这些动态的生物学意义的关键.

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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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