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在尿麻醉小鼠中对连续尿流囊泡测量和内脏运动对膀膨胀的反应进行综合评估

Eric Woon¹, Shaopeng Zhang², Longtu Chen²

¹Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut; UConn Center on Aging, School of Medicine, UConn Health, Farmington, Connecticut; Department of Surgery, University of Connecticut School of Medicine, Farmington, Connecticut.

The Journal of pharmacology and experimental therapeutics

|May 16, 2025

概括

一个新的尿麻醉方案使成熟和老年小鼠的膀功能和疼痛研究能够强大. 这种方法准确地测量了囊泡测量仪和内脏运动反应,推动了膀疾病研究.

科学领域:

生理学生理学生理学
神经科学是一个神经科学.
药理学药理学是指药理学的学科.

背景情况:

膀疾病的研究是使用cystometrogram (CMG) 和内运动反应 (VMR) 在老鼠.
鼠标模型越来越多地用于遗传研究,但麻醉挑战阻碍了老鼠研究.
优化的麻醉对于老年小鼠可靠的膀生理学和疼痛评估至关重要.

研究的目的:

优化尿麻醉方案,在成熟和老年雌性小鼠中进行强大的CMG和VMR记录.
使用此协议来评估膀功能和疼痛反应.
研究乙酸和利多卡因对膀活动和疼痛的影响.

主要方法:

在手术过程中,在最初的异黄素麻醉后,开发了一种连续的尿输注协议.
记录了CMG (肠道压力,排尿量) 和VMR到尿张 (UBD).
静脉注射乙酸以诱导膀刺激和疼痛,随后服用利多卡因以评估缓解疼痛.

主要成果:

优化的尿协议在成熟和老年小鼠中产生了强大的2小时CMG和VMR记录.
酸显著增加了VMR到UBD,并破坏了排尿周期.
利多卡因正常化了被破坏的排尿周期,并减少了由酸诱导的疼痛反应.

结论:

优化的尿麻醉方案有效地研究成熟和老年小鼠的膀生理学和疼痛.
该模型为膀疾病和疼痛的机制研究提供了一个可靠的平台.
这些发现强调了这个模型在评估治疗膀功能障碍和疼痛的治疗干预措施中的实用性.

关键词:

囊管测量图 (Cystometrogram) 是一个测量图.囊泡测量术 (Cystometry) 是一种用于测试囊泡的方法.内性内性膀膨胀是尿道的扩张.视觉运动反应的响应

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