在堆肥过程中通过纳米减少抗生素耐药性基因的机制:洞察宿主微生物和一个双组件系统
Xi Chen1, Muqing Zhan1, Haochen Shao2
1College of Agriculture, Guizhou University, Guizhou Province, Guiyang, 550025, China.
Journal of environmental management
|May 16, 2025
概括
将纳米添加到牛堆肥中有效地减少了抗生素耐药性基因 (ARG). 高度的纳米抑制了转化酶的活性,并削弱了宿主细菌和ARG之间的联系,降低了环境暴露风险.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 农业科学 农业科学
背景情况:
- 纳米 (nano-Se) 与无机相比提供了更好的生物活性,使其成为有前途的农业肥料.
- 抗生素耐药性基因 (ARG) 是一个日益严重的环境问题,特别是在有机废物堆肥过程中.
- 了解纳米Se对ARG及其堆肥中的宿主的影响对于降低风险至关重要.
研究的目的:
- 研究不同纳米-Se度在空气性牛堆肥过程中对ARG和宿主微生物的影响.
- 确定纳米-Se在ARG和移动遗传元素 (MGE) 的丰富性和水平转移中的作用.
- 阐明纳米-Se影响ARG动态和宿主微生物关联的机制.
主要方法:
- 牛的有氧堆肥具有不同的纳米-Se度 (CK,Se_L,Se_M,Se_H).
- 使用分子技术量化ARG和MGE的量化.
- 分析宿主微生物群落 (蛋白质细菌,动态细菌) 和它们对纳米-Se的耐受性.
- 网络分析,Mantle测试和结构方程建模以评估关系.
主要成果:
- 高度的纳米-Se显著降低了占主导地位的ARG的丰度 (例如,阿达达,tetA(48).
- 纳米-Se抑制了转化酶活性,一个关键的MGE,从而降低了抗生素流出ARGs的水平传播风险.
- 主要的ARG宿主 - - Actinobacteria对纳米-Se的耐受性值较低,而高的纳米-Se度会削弱宿主-ARG的关联.
- 结构方程模型表明,纳米-Se主要通过刺激宿主微生物中的信号传导来调节ARG转移.
结论:
- 适当度的纳米-Se有效地降低了牛堆肥期间的ARG水平.
- 纳米-Se通过调节转体酶活性和影响宿主微生物群落来减轻ARG暴露风险.
- 这项研究强调了纳米Se作为在农业废物管理中管理抗生素耐药性的潜在工具.
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