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相关概念视频

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Updated: May 9, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
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整合性表征MYCRNA结合功能的整合性表征

Sihan Li1, Zehua Wang1, Xiaofei Wang1

  • 1Center for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA, USA.

Cell genomics
|May 16, 2025
PubMed
概括

MYC蛋白与许多RNA结合,特别是那些富含guanosine的RNA. 这种RNA结合对MYC至关重要.

关键词:
这就是CRISPR显示器.我的世界 MYC这是一种RNA结合蛋白 (RNA-binding protein,RBP).结合TFRNA的结合方式富含氨酸的图案是丰富的eCLIP eCLIP 是一个增强RNA的RNA增强剂 增强RNA的增强剂基因调节 基因调节 基因调节富含瓜诺辛的RNA是什么?rChIPIP 这是一个很好的例子.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 癌症生物学 癌症生物学

背景情况:

  • MYC基蛋白是一种已知调节基因表达,细胞增殖和新陈代谢的转录因子.
  • 新出现的证据表明,MYC在与RNA分子相互作用方面发挥了作用,但其机制和功能意义在很大程度上仍未被探索.

研究的目的:

  • 为了全面描述MYC作为一种RNA结合蛋白.
  • 阐明MYC-RNA相互作用对MYC生物活动的功能后果.
  • 确定MYC内负责RNA结合的特定区域.

主要方法:

  • 在六个细胞系中对MYC-RNA相互作用的整合性表征.
  • RNA枯竭实验,以评估对MYC染色体占用率的影响.
  • 在体外和体内测试测绘RNA结合域并评估功能后果.
  • 位点定向突变发生,以调查特定氨基酸残留物 (KRR和RQRR动机) 在RNA结合中的作用.

主要成果:

  • MYC与广泛的RNAs结合,更喜欢富含guanosine的序列.
  • 细胞RNAs的枯竭显著降低了MYC与染色素的结合.
  • 在MYC的基本区域中,特定的保存基因 (KRR和RQRR) 对于RNA结合至关重要.
  • 突变KRR基因在体外和体内消除了RNA结合,而不会影响DNA结合.
  • 丢失RNA结合功能会损害MYC的染色体协会,基因激活,细胞循环进展和增殖.

结论:

  • MYC作为一种直接的RNA结合蛋白,具有介导这种相互作用的特定基因.
  • RNA结合是MYC的染色质占用率及其瘤性功能的关键决定因素.
  • 这些发现揭示了MYC活动的新调节层,并为针对MYC驱动的癌症的治疗策略开辟了新的途径.