一个芯片上的丸微流体器官,模仿精子生成表皮
Yanqing Li1, Haicheng Cheng2, LinYan Lv2
1Reproductive Centre；Biomedical Innovation Center, Sun Yat-sen University Sixth Affiliated Hospital, Erheng Road, Yuancun, Tianhe District, Guangzhou City, Guangzhou, --- Select One ---, 510655, CHINA.
Biomedical materials (Bristol, England)
|May 16, 2025
概括
研究人员使用干细胞和支持细胞开发了一种微流体丸器官在芯片上的器官 (OoC). 这个模型成功地模仿丸功能,可以用于毒性测试.
科学领域:
- 生殖生物学 生殖生物学
- 生物工程是生物工程.
- 毒理学 毒理学 毒理学
背景情况:
- 丸精子生殖表皮对于精子发育至关重要,涉及塞尔托利细胞,精子生殖细胞和莱迪格细胞 (LCs).
- 了解丸生理学和查生殖毒素需要有效的模型.
研究的目的:
- 开发一个微流体丸器官芯片器官 (OoC) 模型.
- 评估OOC在模仿丸精子生殖表皮的功能.
- 评估OOC在毒理学查中的潜力.
主要方法:
- 使用精子干细胞,塞尔托利细胞和莱迪格细胞 (LC) 构建了一个微流体装置.
- 在28天内培养丸OoC.
- 应用生殖毒性因子到开发的OOC模型.
主要成果:
- 丸 OoC 形成了一个功能性的精子产生的上皮结构.
- 在OoC中观察到精子干细胞的增殖和分化.
- 塞尔托利细胞和LC维持细胞功能和细胞间通信.
- 暴露于毒性因素减少了精子干细胞的增殖.
结论:
- 微流体丸OoC有效地模拟了丸精子生成表皮.
- 这个OOC平台对研究丸生理学有很大的希望.
- 该模型适用于对生殖健康因素的药理和毒理查.
相关概念视频
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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
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The spermatogenic cells, responsible for producing sperm, are...
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