相关实验视频

Updated: Feb 28, 2026

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

LDL通过PPAR通路调节肠道干细胞稳态

Ruicheng Shi¹, Wei Lu¹, Zhiming Zhao¹

¹Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Journal of lipid research

|May 16, 2025

概括

超脂血症是一种高血脂的状况,促进肠道干细胞 (ISC) 的增殖和干性. 针对PPAR途径可以逆转这些影响,为肠道癌症的发展提供新的见解.

科学领域:

胃肠道学和瘤学
分子生物学分子生物学
代谢疾病代谢疾病

背景情况:

流行病学研究将高脂血症与肠道癌症风险增加联系起来.
肠道干细胞 (ISC) 是肠道瘤发生的起源.
超脂血症对ISC平衡的影响还不清楚.

研究的目的:

为了研究超脂血症对肠干细胞 (ISC) 恒温的影响.
阐明超脂血症影响ISCs的分子机制.
探索与高脂血症相关的肠道瘤发生的潜在治疗点.

主要方法:

利用LDL受体 (Ldlr) 缺乏的小鼠在体内诱导高脂血症.
用LDL活体处理的肠道器官,以评估对ISC干度和生存的影响.
研究过氧体增殖器激活受体 (PPAR) 途径及其下游标 (CPT1A,PDK4) 的作用.

主要成果:

由Ldlr缺乏引起的高脂血症在体内增强了ISC的扩散.
LDL 治疗降低了有机体生存率,但增加了 ISC 干性 ex vivo,由球状体形成和自我更新表明.
LDL激活了PPAR通路;抑制PPAR及其标 (CPT1A,PDK4) 逆转了LDL对ISC的影响.

结论:

超脂血症显著调节肠道干细胞 (ISC) 的稳态.
由LDL诱导的PPAR通路的激活在改变ISC行为中起着关键作用.
准PPAR途径可能提供一个策略,以减轻高脂血症驱动的肠道瘤发生.

关键词:

这些ISC是ISC.这就是为什么LDL,LDL是LDL.这是一个PPAR.脂肪酸氧化过程中的脂肪酸氧化.过高脂血症是什么意思

更多相关视频

The Organoid Reconstitution Assay ORA for the Functional Analysis of Intestinal Stem and Niche Cells

The Organoid Reconstitution Assay ORA for the Functional Analysis of Intestinal Stem and Niche Cells

Published on: November 20, 2017

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Adult Stem Cells

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...

Tissue Renewal without Stem Cells

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...