来自特定乳酸细菌的细胞外囊泡对甲基受体2具有对抗性活性
Hirosuke Sugahara1, Keitaro Nagayama1, Koichi Sashihara1
1Core Technology Laboratories, Asahi Quality and Innovations Ltd., Midori 1-Chome, Moriya-Shi, Ibaraki 302-0106, Japan.
Journal of bioscience and bioengineering
|May 16, 2025
概括
来自特定乳酸细菌 (LAB) 菌株的细胞外囊泡 (EV) 激活了甲基受体2 (FPR2). 这种相互作用是它们抗炎作用和治愈UVB损伤皮肤的能力的关键.
科学领域:
- 食品微生物学 食品微生物学
- 免疫学 免疫学 免疫学
- 皮肤病学 皮肤病学
背景情况:
- 乳酸细菌 (LAB) 和它们的细胞外囊泡 (EV) 已被认为具有有益的健康作用,包括抗炎性质.
- 然而,LAB-derived EVs的菌株特异性功能差异阻碍了对其机制的充分理解.
- 研究这些菌株特异性功能对于利用它们的治疗潜力至关重要.
研究的目的:
- 为了研究LAB衍生的EVs的菌株特异性功能.
- 确定特定受体在调解这些EVs作用中的作用.
- 在皮肤损伤模型中评估LAB衍生的EVs的治疗潜力.
主要方法:
- 评估了来自不同LAB菌株的EVs诱导M2类巨细胞中介质素-10 (IL-10) 分泌的能力.
- 使用G蛋白结合受体 (GPCR) 测试来识别受体相互作用.
- 测试了EVs和FPR2对抗剂对UVB诱导的角质细胞损伤的影响.
主要成果:
- 来自特定LAB菌株的EV显示出对甲基受体2 (FPR2) 的激动性.
- 通过LAB EVs诱导IL-10与它们的FPR2激动活性之间发现了显著的相关性.
- 用FPR2抗剂治疗阻断了IL-10分泌,并逆转了EVs对UVB损坏的角质细胞的修复作用.
结论:
- 特定的LAB衍生的EV具有FPR2的agonist活性,代表了一种新的作用机制.
- 通过LAB EVs激活FPR2是它们抗炎和皮肤保护作用的关键因素.
- 这项研究阐明了LAB EVs的菌株特异性功能,为有针对性的治疗应用铺平了道路.
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