一种与表面显示抗原的重组BCG诱导幽默和细胞免疫反应
Jin-Yu Zhang1, Zhi-Dong Hu2, Li-Xiao Xing1
1Key Laboratory of Medical Molecular Virology of the Ministry of Education/National Health Commission, School of Basic Medical Sciences and Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
Scientific reports
|May 16, 2025
概括
在Bacillus Calmette-Guérin (BCG) 重组疫苗的细胞壁上显示抗原,可增强长期中和抗体 (Nab) 生产和T细胞反应,为改进的疫苗平台提供了有希望的策略.
科学领域:
- 疫苗学 疫苗学 疫苗学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 卡尔梅特-盖林杆菌 (Bacillus Calmette-Guérin,简称BCG) 是一种广泛使用的结核疫苗.
- 作为传递抗原的载体,BCG正在被探索,但在诱导持久免疫反应方面面临挑战,特别是中和抗体 (Nabs).
研究的目的:
- 研究在BCG细胞壁上显示抗原的有效性,以诱导强大且持久的免疫反应.
- 为了评估表达SARS-CoV-2受体结合域 (RBD) 抗原在其细胞壁上的重组BCG (CW-rBCG::RBD).
主要方法:
- 在细胞壁上表达SARS-CoV-2 RBD抗原的重组BCG (CW-rBCG::RBD) 的构造.
- 在小鼠中通过静脉和皮下途径给予CW-rBCG::RBD,与父母BCG和RBD蛋白子单位疫苗 (RBDAS01) 进行比较.
- 在不同时间点评估中和抗体 (Nab) 标位,T细胞反应 (Tfh,Tcm) 和抗体同型 (IgG2a).
主要成果:
- CW-rBCG::RBD显示了高抗原产量.
- 静脉注射与助推剂一起显著增强了NAB生产和T细胞反应 (Tcm,Tfh).
- 用CW-rBCG::RBD接着RBDAS01的单剂量启动诱导了NABS和IgG2a抗体的升高,持续了8周. 皮下同时使用31周维持了NABS,并增强了Tfh/Tcm细胞.
结论:
- 在BCG细胞壁上显示抗原是增强疫苗诱导免疫反应的有效策略.
- 这种方法有望优化BCG基础的疫苗接种和免疫治疗平台,用于传染病和癌症.
- 这项研究强调了一种提高BCG疫苗免疫性和耐久性的新方法.
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