相关实验视频
Updated: May 8, 2026
11:00
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
在植物中预测miRNA目标的预测
Shree P Pandey1, Priyanka Pandey2, Prashant K Srivastava3
1College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China. shreepp@lzu.edu.cn.
Methods in molecular biology (Clifton, N.J.)
|May 16, 2025
概括
这项研究介绍了一种计算方法,用于预测植物中的microRNA (miRNA) 目标. 该方法有助于理解基因调节,特别是在缺乏专门工具的非模型植物物种中.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因调节 基因调节
- 生物信息学是一种生物信息学.
背景情况:
- 微RNAs (miRNAs) 是植物在转录后水平上基因表达的关键调节者.
- 在植物中,miRNA目标识别通常涉及高序列互补性.
- 对于miRNA目标预测的现有计算工具主要是为模型植物开发的,限制了对非模型物种的分析.
研究的目的:
- 开发一种分析植物miRNA介导基因调节的计算程序.
- 为了促进miRNA目标的全基因组预测,特别是在非模型植物物种中.
- 了解由miRNAs及其目标形成的复杂的调节网络.
主要方法:
- 这项研究采用了基于miRNA生物学既定原则的计算方法.
- 它利用了miRNAs及其目标转录之间的高序列互补性.
- 该程序考虑了miRNAs对目标基因表达和潜在网络形成的负面调节作用.
主要成果:
- 描述了一种用于预测植物miRNA点的新型计算程序.
- 该方法适用于全基因组分析,解决非模型物种的挑战.
- 它为研究miRNA介导的相互作用和调节网络提供了一个框架.
结论:
- 开发的计算程序为植物miRNA研究提供了有价值的工具.
- 它增强了在各种植物物种中通过miRNAs调节基因调节的研究能力.
- 这项工作有助于更深入地了解植物王国的转录后基因调节.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...