相关实验视频

Updated: May 4, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 4, 2010

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具有成本效益和灵敏的基于探针的通用 (PBU) qPCR用于植物中的微RNA量化

Pedro Fernando Vera Hernández1, Catarino Eduardo Téllez Valerio2, Flor de Fátima Rosas Cárdenas3

  • 1Universidad Autónoma del Estado de México, Toluca, Estado de México, Mexico.

Methods in molecular biology (Clifton, N.J.)
|May 16, 2025
PubMed
概括

这项研究引入了一种具有成本效益的,基于探针的实时RT-PCR方法,用于量化植物微RNA (miRNA). 这种敏感的技术能够对植物小RNA进行准确的分析,这对于理解发育和环境反应至关重要.

关键词:
有成本效益的方法.微RNAs是一种微RNA.植物miRNA分析分析基于探针的qPCR检测量化 量化 量化 量化实时PCR可以实现.全球性的 qPCRR.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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