化疗提高了细胞表面PD-L1和MHC-I表达在胃癌细胞的胃癌细胞
You-Syuan Lou1, Xu-Chen Liu1, Chih-Cheng Cheng2
1School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
概括
化疗增加了编程细胞死亡连接体1 (PD-L1) 和主要组织相容性复合体I类 (MHC-I) 在胃癌细胞上,主要是在亡细胞上. 这些发现影响了抗PD-1/PD-L1治疗患者的选择.
科学领域:
- 在瘤学瘤学.
- 免疫治疗是一种免疫疗法.
- 癌细胞生物学 癌细胞生物学
背景情况:
- 编程细胞死亡连接体1 (PD-L1) 和主要组织相容性复合体I类 (MHC-I) 表达对于胃癌中抗编程细胞死亡-1 (PD-1) /PD-L1治疗至关重要.
- 传统治疗可以改变PD-L1和MHC-I的表达,影响治疗疗效.
研究的目的:
- 研究化疗对PD-L1表面和MHC-I表达在活细胞和胃癌细胞的影响.
- 分析各种化疗剂在不同胃癌细胞系中对PD-L1和MHC-I表达的差异效应.
主要方法:
- 胃癌细胞系 (AGS和SNU-1) 接受了使用5-Fluorouracil (5-Fu),cisplatin,mitomycin c (MMC) 和FOLFOX的治疗.
- 流细胞测量被用来量化表面PD-L1和MHC-I表达在共染后的活细胞 (附素V阴性) 和亡细胞 (附素V阳性) 上.
主要成果:
- 所有测试的化疗剂都在AGS和SNU-1细胞中增加了表面PD-L1和MHC-I水平,增加程度各不相同.
- 5-Fluorouracil (5-Fu) 在AGS细胞中诱导了PD-L1和MHC-I的最显著的上调,但在SNU-1细胞中最少.
- 化疗诱导的PD-L1和MHC-I的增加主要在胃癌细胞上被观察到.
结论:
- 化疗主要增强在胃癌细胞上的表面PD-L1和MHC-I表达.
- 这些发现表明,化疗可能调节瘤微环境和胃癌免疫反应的机制.
更多相关视频
10:28Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Published on: January 22, 2018
10.7K
10:11Immunohistochemical Staining of B7-H1 PD-L1 on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue
Published on: January 3, 2013
23.0K
相关概念视频
01:31The Intrinsic Apoptotic Pathway
5.8K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
5.8K
01:17Phagocytosis of Apoptotic Cells
3.2K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.2K