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相关概念视频

Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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相关实验视频

Updated: Jun 19, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

核糖体分析技术的进展 核糖体分析技术的进展

Kotaro Tomuro1,2, Shintaro Iwasaki1,2

  • 1RNA Systems Biochemistry Laboratory, Pioneering Research Institute, RIKEN, Wako, Saitama 351-0198, Japan.

Biochemical Society transactions
|May 17, 2025
PubMed
概括
此摘要是机器生成的。

核糖体造型 (Ribo-seq) 揭示了使用核糖体足迹调节蛋白质合成的情况. 最近的Ribo-seq创新提高了灵敏度和分辨率,以获得详细的翻译控制见解.

关键词:
深度测序是一种深度测序.里波-seqq 的意思.核糖体造型分析是指核糖体造型.翻译控制的翻译控制

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

相关实验视频

Last Updated: Jun 19, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 核糖体分析 (Ribo-seq) 是研究蛋白质合成的一个关键技术.
  • 核糖体足迹的深度测序为转化调节提供了洞察力.
  • 在Ribo-seq的进步提高了它的灵敏度,特异性和分辨率.

研究的目的:

  • 审查新兴的Ribo-seq衍生品及其应用.
  • 突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出
  • 为了展示Ribo-seq在理解翻译控制方面的实用性.

主要方法:

  • 关于Ribo-seq技术的最新文献的审查.
  • 对Ribo-seq衍生品的分析,以解决特定的挑战.
  • 讨论各种生物环境中的应用.

主要成果:

  • 新兴的Ribo-seq方法解决了诸如低输入,rRNA污染和数据校准等挑战.
  • 新的Ribo-seq技术支持单细胞应用.
  • 这些进展为翻译法规提供了更深入的见解.

结论:

  • Ribo-seq是研究蛋白质合成的强大和不断发展的工具.
  • 在Ribo-seq的创新正在扩大其范围和适用性.
  • 里博-seq衍生品为剖析翻译控制提供了增强的能力.