基于基因组的临床警报来自泰国的Plesiomonas shigelloides PSU59:耐药性和毒性特征
Thunchanok Yaikhan1, Kamonnut Singkhamanan1, Thitaporn Dechathai1
1Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.
概括
来自泰国的细菌Plesiomonas shigelloides PSU59,显示出作为新兴病原体的潜力. 基因组分析揭示了抗微生物耐药性基因和毒性因素,突出显示了监测的必要性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- Plesiomonas shigelloides是一种水生细菌,作为一种新兴的病原体得到了认可.
- 抗菌素耐药性 (AMR) 是P. shigelloides感染中越来越令人担忧的一个问题.
研究的目的:
- 为了进行基因组基因分析P. shigelloides PSU59,从泰国的一名患者中分离出来.
- 为了确定P. shigelloides PSU59基因组中的AMR和毒性因素.
主要方法:
- P. shigelloides的全基因组测序 (WGS) PSU59.9.
- 生物信息分析用于识别编码序列,RNA基因,AMR基因和毒性因子.
- 与其他P. shigelloides菌株的遗传学比较.
主要成果:
- 测序了一份3.6Mb的基因组草案,其中包含3265个编码序列和129个RNA基因.
- 确定了13个AMR基因,包括排泄和标修饰剂.
- 检测到48种毒性因素,包括与机动性相关的鞭毛基因. 移动遗传元素表明水平基因转移.
结论:
- P. shigelloides PSU59具有显著的致病性和耐药性潜力.
- 基因组监测对于监测P. shigelloides等未被认可的病原体带来的新兴威胁至关重要.
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