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酵母复制翻译试验用于分析eIF5A功能的分析

Byung-Sik Shin1, Thomas E Dever1

  • 1Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States.

Methods in enzymology
|May 17, 2025
PubMed
概括

多氨酸对蛋白质合成至关重要,特别是转化因子eIF5A,这对于延长和终结至关重要. 提出了新的生化分析,以研究eIF5A调节及其在神经发育障碍中的作用.

关键词:
在 DHPS 系统中使用 DHPS.这就是为什么DOHH DOHH.延伸 延伸 延伸 延伸 延伸下垂体是指下垂体的一个部分.重建的翻译重建的翻译.核糖体 核糖体 核糖体 核糖体终止 终止 终止 终止 终止酵母酵母是一种酵母.在 eIF5A 中,eIF5A 是

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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