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Updated: May 20, 2025

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
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通过LC-MS使用异布基甲酸衍生物进行聚胺量化

Christine Isaguirre1, Megan Gendjar1, Kelsie M Nauta2

  • 1Mass Spectrometry Core, Van Andel Institute, Grand Rapids, MI, United States.

Methods in enzymology
|May 17, 2025
PubMed
概括

本研究介绍了一种简单的异布基酸盐衍生法,用于使用液态染色体质谱法量化聚氨酸. 该协议通过准确测量十三种多氨酸,甚至共释放异构体,从而增强新陈代谢.

关键词:
乙精氨酸是什么?代谢过程中的代谢.代谢学 代谢学 代谢学逆转了相位的阶段.

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相关概念视频

Mass Spectrometry of Amines01:19

Mass Spectrometry of Amines

In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule: a molecule with an odd number of nitrogen atoms produces a parent ion with an odd molecular weight. The remaining fragments have an even mass.
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
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