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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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相关实验视频

Updated: Jun 30, 2026

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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来自中国软海 (Pelodiscus sinensis) 的数字网络之间的转录组数据.

Shibin Bai1, Shanshan Li1, Yining Tang1

  • 1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.

Scientific data
|May 17, 2025
PubMed
概括

研究人员研究了中国软海网络发展中的基因表达,以了解水生适应如何演变. 这项研究揭示了四肢形态发生和数字间细胞死亡的调节.

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科学领域:

  • 发展生物学 发展生物学
  • 进化生物学 进化生物学
  • 进行比较基因组学.

背景情况:

  • 水生四足动物的指际网对于适应至关重要,它是由受抑制的指际细胞死亡 (ICD) 引起的.
  • 多种不同的网络形态学已经独立发展,但基本的监管机制仍然不清楚.
  • 中国软海 (Pelodiscus sinensis) 显示过渡性网状,使其成为一个理想的模型生物体.

研究的目的:

  • 调查调控Pelodiscus sinensis.数字间的发展的分子机制.
  • 识别关键基因,参与肢体形态发生过程中的数字间细胞死亡 (ICD) 的调节.
  • 为四足动物水生适应的融合进化提供见解.

主要方法:

  • RNA测序 (RNA-seq) 在TK19.9阶段的胚胎Pelodiscus sinensis的8个数字间网络样本上进行.
  • 在样本之间确定了差异表达基因 (DEG).
  • 整体在位杂交 (WISH) 和实时定量PCR (RT-qPCR) 用于验证转录组发现.

主要成果:

  • 在正在发展的数字网络中,共发现608个差异表达基因 (DEG).
  • 使用WISH和RT-qPCR的验证证实了RNA-seq数据对所选基因的准确性.
  • 该研究确定了ICD和网络形成的潜在遗传调节者.

结论:

  • 这项研究为了解四足动物水生适应和四肢发育的演变提供了有价值的转录基因数据.
  • 这些已识别的基因为研究编程细胞死亡和衰老的可塑性提供了潜在的分子标.
  • 这些发现有助于比较基因组学和适应性融合进化的研究.