从血滴到生物标志物:在基于质谱的蛋白质组学中对微采样进行范围审查
Amanda J Campbell1,2,3, Nicolai B Palstrøm1,2,3, Lars M Rasmussen1,2,3,4
1Department of Clinical Biochemistry, Odense University Hospital, Odense, Denmark.
Clinical proteomics
|May 18, 2025
概括
微样本显示出临床蛋白质组学的前景,提供更容易的血液采集. 然而,需要标准化量化方法才能充分发挥它们在大规模研究中的潜力.
科学领域:
- 临床蛋白质组学 临床蛋白质组学
- 基于质谱的蛋白质组学.
- 微采样技术的微采样技术
背景情况:
- 在蛋白质组学中,从小血液抽取中获得的微样本正在出现.
- 与传统的血或血清样本相比,微样本的当前蛋白质组分析在定量精度和覆盖率方面面临挑战.
- 这限制了它们在蛋白质组学研究中的广泛采用.
研究的目的:
- 审查微样本在基于临床质谱的蛋白质组学中的应用.
- 评估使用微样本的概念验证和临床研究.
- 用微型样本评估临床蛋白质组学的最新进展.
主要方法:
- 用自下而上的质谱法对人类微样本内源性蛋白质分析的科学文献进行范围审查.
- 搜索了MEDLINE,EMBASE和Scopus数据库,并通过引用搜索进行了补充.
- 遵守系统性审查和元分析的优先报告项目,扩展范围审查 (PRISMA-ScR) 的指导方针.
主要成果:
- 在对数千份记录进行选后,有64项研究符合条件.
- 微样本适用于高通量蛋白质组学,但量化仍然是一个挑战.
- 指出了异质的微样采样实践和缺乏标准化量化程序.
- 临床研究探讨了各种疾病群体中的蛋白质表达,包括血红蛋白病和免疫缺陷障碍.
结论:
- 微样本在蛋白质组学中越来越多地使用,并且与标准的自下而上的工作流程兼容.
- 微样采集和量化程序的标准化是必要的.
- 解决这些问题可以显著减少对临床蛋白质组学的大型队列研究的资源需求,目前缺乏足够的发现和验证队列.
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