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长读测序用于对传染病爆发的快速反应

Josephine B Oehler^1,2, Kaitlin Burns^2,3, Jeffrey Warner^2,3

¹Computational Biomedicine Lab, College of Science and Engineering, James Cook University, 1 James Cook Drive, Townsville, QLD 4811 Australia.

Current clinical microbiology reports

|May 19, 2025

概括

长读测序 (LRS) 为实时疫情响应提供了高可靠性病原体基因组分析. 本综述涵盖了LRS的进展,流行病学和公共卫生中的应用以及未来的整合策略.

科学领域:

基因组流行病学基因组流行病学
病原体监测病原体监测病原体监测
生物信息学是一种生物信息学.

背景情况:

长读测序 (LRS) 提供了对传染病爆发反应至关重要的高可靠性基因组数据.
牛津纳米孔技术 (ONT) 和太平洋生物科学 (PacBio) 等LRS技术使复杂的基因组结构和抗菌素耐药性的详细分析成为可能.
在埃博拉和COVID-19等全球卫生危机期间,LRS设备的便携性是基于现场的病原体识别的关键.

研究的目的:

审查长读序列 (LRS) 技术及其应用的最新进展.
评估LRS对基因组流行病学,元基因组学和公共卫生决策的影响.
确定将LRS整合到全球疫情监测系统中的局限性和未来前景.

主要方法:

在病原体监测中对长读测序的最近研究的文献综述.
在基因组流行病学和转基因组学中对LRS应用的研究结果的综合.
对LRS性能进行批判性评估,包括准确性,可移植性和限制.

主要成果:

与短读测序相比,LRS提供了复杂的基因组特征,抗菌素耐药性基因和传播动态的卓越分辨率.
便携式LRS平台在重大疫情期间能够快速地在现场识别病原体.
持续的挑战包括测序错误率,计算需求和成本效益.

结论:

长读测序是病原体监测和疫情应对的变革性工具.
解决目前的局限性对于最大限度地发挥LRS在全球卫生安全方面的潜力至关重要.
利用LRS知识对于研究人员和公共卫生专业人员来说至关重要,他们面临着不断变化的传染病威胁.

关键词:

基因组流行病学抗菌素耐药性概况分析转基因组学是指转基因组学.病原体的鉴定病原体的鉴定实时监控实时监控第三代测序系统是第三代测序系统.

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