LC-MS/MS指纹分析Cyanotis arachnoidea提取物:与合成代谢食品补充剂中的工艺相关人工物
Dávid Laczkó1,2, En-Liang Chu3, Ching-Chia Chang4
1Institute of Pharmacognosy, University of Szeged, Szeged H-6720, Hungary.
在Cyanotis arachnoidea中,20-hydroxyecdysone (20E) 的自氧化会产生未知化合物,影响补充剂的质量和安全性. 这项研究确定了146种ecdysteroids,澄清了加工对商业补充剂的影响.
科学领域:
- 植物化学 植物化学
- 自然产品化学 自然产品化学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 乙类固醇是植物衍生化合物,具有多样化的药理活性.
- 来自Cyanotis arachnoidea的商业补充剂可能含有降解产品.
- 自氧化20-基乙 (20E) 可以导致不具特征的化合物.
研究的目的:
- 从Cyanotis arachnoidea中分离和表征新的生态类固体.
- 在植物提取物和自氧化混合物中进行ecdysteroids的LC-MS/MS指纹检测.
- 评估自氧化对商业生态类固醇补充剂的质量和安全性的影响.
主要方法:
- 隔离和完整的核磁共振 (NMR) 四个新的ecdysteroids的特征.
- 液体染色学-并联质谱学 (LC-MS/MS) 指纹.
- 对原生和经过加工的Cyanotis arachnoidea提取物和自氧化20-hydroxyecdysone (20E) 混合物的分析.
主要成果:
- 四种新的ecdysteroids被隔离和完全表征.
- 在分析的样本中总共检测到146种ecdysteroids.
- 使用参考标准确定了20种ecdysteroids,包括最近报告的化合物.
- 自氧化降解产物被证实在商业生态类固醇提取物中.
结论:
- 自氧化显著改变了Cyanotis arachnoidea提取物中的ecdysteroid形状.
- 从自氧化过程中产生的与过程相关的工件可能会影响生态类固醇补充剂的有效性和安全性.
- 了解这些转变对于质量控制和监管评估含有ecdysteroid的产品至关重要.
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