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外体 miR-182-5p 是一种潜在的诊断标志物,用于恶性外泄

Wen Zhao^1,2,3, Jian-Xun Wen^3,4, Yan Niu^3,4

¹Center for Clinical Epidemiology Research, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

Translational lung cancer research

|May 19, 2025

概括

外体微RNAs (miRNAs) 显示出作为生物标志物用于诊断恶性流 (MPE) 的前景. 外体miR-182-5p可以帮助MPE诊断,其性能略受使用的内部参考因素的影响.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

恶性多叶流血 (MPE) 诊断可能具有挑战性.
外体微RNA (miRNA) 正在成为各种疾病的潜在生物标志物.
外体miRNAs在诊断MPE中的实用性需要进一步调查.

研究的目的:

分析MPE和良性肺溢液 (BPE) 中的外体miRNA配置文件.
为了评估外体miRNAs对MPE的诊断性能.
确定外体miRNA是否可以提高现有标记物的诊断准确性,例如癌胚抗原 (CEA).

主要方法:

下一代测序 (NGS) 用于在MPE和BPE样本中对外体miRNA进行分析.
使用逆转录聚合酶链反应 (RT-PCR) 验证了差异表达的miRNA.
接收器操作特征 (ROC) 曲线分析评估了诊断准确性,并评估了与CEA的组合.

主要成果:

发现大量的miRNAs在MPE与BPE相比被差异地表达.
异位体miR-182-5p被确定为MPE的潜在诊断标记物.
外体miR-182-5p在与CEA (AUC 0.91) 结合时,表现出良好的诊断准确性 (AUC 0.78-0.80) 和略有改善的MPE诊断.

结论:

腹液外体miR-182-5p可以作为MPE诊断的辅助生物标志物.
选择内部参考 (cel-miR-39或snRNA U6) 可能会对外体miR-182-5p的诊断性能产生轻微影响.
将外体miR-182-5p与CEA结合起来,可以提高MPE的诊断准确性.

关键词:

恶性肺溢液 (MPE) 是一种恶性肺溢液.诊断的准确性诊断的准确性这是一种外体miRNA.在 miR-182-5p 中.

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