POSTN沉默改善LL37-诱导的粉红,并抑制了JAK2/STAT3和NF-κB通路
Yan Chen1, Yang Gao2, Zhang Zhang3
1Department of Dermatology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
概括
皮洛斯 (POSTN) 降低显著降低了粉红病小鼠模型中的炎症和血管生长. 这表明POSTN沉默可能通过向关键的炎症途径,为粉红病提供一种新的治疗策略.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 红是一种慢性炎症性皮肤疾病,原因不明.
- 识别参与粉红病原发生的关键基因对于开发有效治疗方法至关重要.
研究的目的:
- 为了研究皮奥斯 (POSTN) 在病发病过程中的作用.
- 为了评估POSTN的治疗潜力,在一个类似于rosacea的小鼠模型中敲击下来.
主要方法:
- 对基因表达数据集的生物信息分析 (GSE155141,GSE65914).
- 使用lentivirus包装的Lv-shPOSTN和LL37治疗方法开发一种类似罗莎的小鼠模型.
- 评估皮肤病变,炎症,免疫细胞透和血管生成.
- 在体外测试 (伤口愈合,Transwell) 和西部涂抹以分析细胞过程和信号通路 (JAK2 / STAT3,NF-κB).
主要成果:
- 皮洛斯 (POSTN) 被确定为粉红病中高度表达的基因.
- 在小鼠中,POSTN knockdown改善了皮肤病变,减少了炎症,免疫细胞透和血管生成.
- POSTN沉默降低了关键炎症因子 (TNF-α,IL-1β,IL-6) 和化学因子 (CCL2,CXCL10,CXCL2) 的水平.
- POSTN knockdown抑制了内皮细胞的迁移和侵入,并在体内和体外抑制了JAK2/STAT3和NF-κB信号通路.
结论:
- 皮洛斯 (POSTN) 在病的发病过程中起着重要作用.
- 通过抑制炎症和血管生成,POSTN Knockdown证明了治疗潜力.
- 通过敲击准POSTN提供了通过调节JAK2/STAT3和NF-κB通路来治疗粉红病的有希望的策略.
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