蛋白质结构和生物活性在纳米持续释放输送装置的吸附和脱附后
Samuel Stealey1, Ether Dharmesh1, Akhilesh K Gaharwar2
1Department of Biomedical Engineering, Saint Louis University, Saint Louis, MO, USA. silviya.zustiak@slu.edu.
Nanoscale
|May 19, 2025
概括
拉波尼特® XLG纳米酸盐 (NS) 通过水凝增强了蛋白质的输送. 虽然蛋白质在与NS复杂化后暂时展开并失去活性,但在从水凝释放后恢复结构和功能,从而使可调节的药物输送成为可能.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物输送系统 药物输送系统
背景情况:
- 使用水凝载体的生物制剂的持续局部输送对于提高蛋白质安全性和有效性至关重要.
- 将纳米颗粒纳入水凝可以改善生物制剂的保留和释放动力学.
- 纳米酸盐,如LAPONITE® XLG (NS),为复杂化生物提供了很大的表面积,使可调节的释放配置文件成为可能.
研究的目的:
- 研究纳米酸盐-蛋白质复合物的结构和稳定性.
- 为了评估与纳米酸盐复合后的蛋白质活性和结构变化.
- 了解蛋白质和纳米酸盐之间的相互作用,用于先进的药物输送应用.
主要方法:
- 结合亲和度测试以确定蛋白质-纳米酸盐相互作用.
- 用光谱方法评估蛋白质结构和展开.
- 生物活性测定测量蛋白质功能后复杂化和释放.
- 在染剂的存在下分析蛋白质稳定性.
主要成果:
- 蛋白质电荷和与NS的结合亲和力之间观察到强烈的相关性,正电荷蛋白质具有更高的吸引力.
- 蛋白质在溶液中表现出暂时的展开和部分生物活性损失与NS.
- 从PEG-NS水凝中释放的蛋白质证明了二次结构和生物活性的恢复.
- 纳米酸盐结合提供了对化剂guanidine thiocyanate的部分保护.
结论:
- 纳米酸盐-蛋白质复合影响蛋白质结构和活性,但在从水凝释放后,这种情况是可逆的.
- 了解这些相互作用是开发基于NS的水凝的关键,以实现可调节,持续的生物传递.
- 这项研究支持使用NS-蛋白质复合物作为先进药物输送装置中有效组件.
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