相关实验视频
Updated: May 20, 2025
11:31
Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
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概括
尿液生物标志物的稳定性随储存而变化. N-乙-β-葡萄糖胺酶 (U-NAG) 和视网醇结合蛋白 (U-RBP) 在-20°C不稳定,尿液pH会影响几个生物标志物.
科学领域:
- 临床化学 临床化学
- 生物标记分析 生物标记分析
- 尿液蛋白质组学 尿液蛋白质组学
背景情况:
- 精确的尿液生物标志物测量对于诊断和监测病至关重要.
- 缺乏用于尿样存储和pH的标准化协议,这可能会影响生物标志物的完整性.
研究的目的:
- 评估各种储存条件 (温度和持续时间) 和pH值对九种关键尿路蛋白质稳定性的影响.
- 为最佳的尿样处理提供基于证据的建议,以确保生物标志物的可靠性.
主要方法:
- 从健康人群和脏病患者收集尿液.
- 在四种储存条件下评估了九种生物标志物的稳定性 (U-α1MG,U-Alb,U-Trf,U-IgG,U-NAG,U-NGAL,U-RBP,U-CysC,U-TP):25°C/8h,4°C/7d,-20°C/12w,-80°C/12m.
- 在4.0至8.5的pH范围内测试了稳定性.
主要成果:
- 在测试条件下,U-Albumin (U-Alb),U-alpha-1-microglobulin (U-α1MG),U-Transferrin (U-Trf),U-Total Protein (U-TP) 和U-Cystatin C (U-CysC) 显示出良好的稳定性.
- 在特定的储存条件和/或pH水平下,U-免疫球蛋白G (U-IgG),U-NAG和U-联蛋白 (U-RBP) 呈现不稳定性.
- 在4°C和-20°C时,U-NAG和U-RBP特别不稳定,它们的稳定性也受到pH极端的影响.
结论:
- 由于显著的不稳定性,U-NAG和U-RBP不应在-20°C储存.
- U-IgG的稳定性是有限的,特别是在长时间的-20°C和-80°C.
- 保持尿液pH值在特定范围内 (例如,某些生物标志物的5.0-7.5) 对于准确的结果至关重要;可能需要调整.
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