序列标记辅助精确和多目标分析细胞外囊上的表面蛋白质.
Xiaomeng Yu1, Ya Cao1, Jianan Xia2
1State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210023, PR China.
Analytical chemistry
|May 19, 2025
概括
这项研究引入了一种新的电化学方法,用于分析细胞外囊泡 (EVs) 上的多重表面蛋白. 这种技术可以准确检测乳腺癌生物标志物,有助于早期诊断和个性化治疗.
科学领域:
- 生物技术是生物技术.
- 分析化学 分析化学
- 癌症研究 癌症研究
背景情况:
- 细胞外囊泡 (EVs) 携带表面蛋白质,对于理解癌症生物学至关重要,特别是在异质乳腺癌中.
- 在EV上评估多个表面蛋白质是具有挑战性的,因为它们的小尺寸和空间障碍.
- 识别特定于癌症的EV表面蛋白可以揭示治疗点和诊断标记.
研究的目的:
- 开发一种新的顺序标记辅助电化学方法,用于对单个EV进行精确的多蛋白质分析.
- 在分析EV表面蛋白质时克服空间障碍的限制.
- 证明该方法在检测乳腺癌生物标志物的实用性及其临床应用潜力.
主要方法:
- 使用用电活性纳米粒子功能化的aptamer探针对EV表面蛋白进行序列标记.
- 使用由白素-Fe2+复合体介导的氧化裂变过程,以实现序列检测.
- 电化学分析用于量化目标蛋白质,如皮肤上生长因子受体和EVs上的编程死亡连接体-1.
主要成果:
- 顺序标记方法有效地减轻了空间障碍,允许在EV上准确检测多蛋白质.
- 该方法在从三阴性乳腺癌 (TNBC) 细胞 (低至341颗粒/毫升) 中低度的标准EVs上实现了目标蛋白的精确量化.
- 从健康个人和TNBC患者的临床血液样本中成功应用,证明了诊断潜力.
结论:
- 开发的方法提供了一个可行的工具,用于对单个EV的表面蛋白质进行精确的多重分析.
- 这种方法为准确的乳腺癌诊断和个性化治疗策略提供了宝贵的蛋白质水平信息.
- 该技术在早期癌症诊断和疾病过程监测方面表现有前途.
更多相关视频
06:12Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
1.3K
06:31"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
Published on: March 24, 2023
2.1K
相关概念视频
01:41Signal Sequences and Sorting Receptors
9.9K
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
9.9K
01:24Tagging and Fusion Proteins
6.4K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.4K