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相关概念视频

Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
Major Organs of the Digestive System01:19

Major Organs of the Digestive System

The digestive system is responsible for the ingestion of food, secretion of enzymes, mixing and digesting food, absorption of the nutrients and defecation. The human digestive system consists of two major parts: the gastrointestinal tract and the accessory digestive organs.
Gastrointestinal tract:
Peritoneum01:21

Peritoneum

The peritoneum is a vital membrane that lines the abdominal cavity and covers most of the organs within it. It plays a crucial role in protecting the organs, providing a smooth surface for their movement, and facilitating various physiological processes. Understanding the anatomy and function of the peritoneum is essential for comprehending the complexities of the abdominal region.
Anatomy of the Peritoneum
The peritoneum is divided into two layers: the parietal peritoneum and the visceral...
Gross Anatomy of the Liver01:17

Gross Anatomy of the Liver

The liver, the largest gland within the human body, is a firm and reddish-brown organ. This wedge-shaped structure weighs approximately 1.5 kg and occupies a significant portion of the right hypochondriac and epigastric regions. It extends more to the right of the body's midline than to the left.
Located under the diaphragm, the liver is almost entirely ensconced within the rib cage, providing it with substantial protection. Except for the superior most bare area, the liver's surface is covered...
Liver Histology01:27

Liver Histology

The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
Hepatocytes perform a variety of essential functions. They secrete...
Liver Physiology01:30

Liver Physiology

The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of  70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can also...

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相关实验视频

Updated: Jul 24, 2026

Generation of Organoids from Mouse Extrahepatic Bile Ducts
09:13

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

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产生功能性内皮性肝脏器官.

Soheil Akbari1, Nevin Ersoy2, Alper Bagriyanik3

  • 1Izmir Biomedicine and Genome Center.

Journal of visualized experiments : JoVE
|May 19, 2025
PubMed
概括

研究人员开发了一种快速的协议,可以从多能干细胞中制造功能性肝脏器官. 这些肝脏器官 (eHEPOs) 模仿肝脏功能,可以长期扩大,为肝细胞提供新的来源.

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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
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科学领域:

  • 干细胞生物学 干细胞生物学
  • 机器人技术是机器人技术.
  • 肝病学 肝病学是一种肝病学.

背景情况:

  • 机器人技术使人体器官类似的微型结构能够在体外生成.
  • 有机体模型的进步对于疾病建模,发育生物学和药物发现至关重要.
  • 诱导多能干细胞 (iPSC) 衍生的肝脏器官是产生肝细胞的关键.

研究的目的:

  • 从多能干细胞生成肝脏器官的强大和可复制的协议.
  • 建立一种快速有效的方法,在两周内制造功能性肝脏器官.
  • 为生产功能性肝细胞提供替代来源.

主要方法:

  • iPSCs直接分化为内皮细胞.
  • 通过FACS丰富EpCAM阳性细胞.
  • 使用膨胀介质建立肝脏器官,以创建3D内皮衍生肝脏器官 (eHEPOs).

主要成果:

  • 肝脏器官 (eHEPOs) 在2周内产生.
  • eHEPOs模仿肝细胞的基本功能,包括白蛋白分泌,糖原储存和细胞染色体P450活性.
  • eHEPOs表现出肝脏特异性基因表达,两极化上皮细胞和胆管,并且可以连续传递长达一年的时间,而不会失去分化能力.

结论:

  • 开发的协议提供了一种快速,高效和可重复的方法,用于从多能干细胞生成功能性肝脏器官 (eHEPOs).
  • eHEPOs作为一种有价值的体外模型,用于研究肝脏生物学和疾病,以及药物发现.
  • eHEPOs的长期扩张和分化能力为功能性肝细胞提供了一个有希望的替代来源.