格拉斯蛋白RAM1与WRI转录因子相互作用,调节树发育和功能所需的植物基因
Michael Paries1, Karen Hobecker1,2, Sofia Hernandez Luelmo1,2
1Plant Genetics, TUM School of Life Sciences, Technical University of Munich (TUM), 85354 Freising, Germany.
概括
树枝状菌根的共生包括营养交换. 需要ARBUSCULAR MYCORRHIZATION1 (RAM1) 蛋白与类似WRINKLED1的转录因子相互作用,以激活这种植物-真菌伙伴关系必不可少的基因.
科学领域:
- 植物与微生物的相互作用
- 分子植物科学 分子植物科学
- 树状菌根的共生体是树状的.
背景情况:
- 树枝状菌根 (AM) 的共生对于植物的营养吸收至关重要.
- 需要ARBUSCULAR MYCORRHIZATION1 (RAM1) 是一种GRAS蛋白,对AM共生至关重要,但其DNA结合机制尚不清楚.
- 了解RAM1的功能是提高植物营养交换的关键.
研究的目的:
- 阐明RAM1在AM共生中激活目标基因的分子机制.
- 确定与RAM1相互作用的蛋白质及其在转录调节中的作用.
- 描述控制关键AM基因表达的调控元素和途径.
主要方法:
- 酵母三混合测试检测蛋白质与蛋白质相互作用.
- 在 *Nicotiana benthamiana* 和 *Lotus japonicus* 中进行记者基因测定,以评估促进子激活.
- 对基因促进体中保存的动机和调节元件的分析.
主要成果:
- RAM1通过其C端M2/M2a动机与五种类似WRINKLED1的APETALA 2转录因子 (CBX1,WRI3,WRI5a,WRI5b,WRI5c) 相互作用.
- RAM1 和 WRI 蛋白合作激活由 * MYCS * 元素和 * AW * 盒子所依赖的共生营养交换基因 (* RAM2 * * STR * * * PT4 *) 的促进者.
- *WRI*基因的表达以两个波调节,其中包括CYCLOPS和DELLA,以及RAM1-WRI异构体.
结论:
- RAM1充当转录协激活剂,为DNA结合的WRI转录因子提供激活域.
- 这种RAM1-WRI复合体对于激活关键基因至关重要,因为它对ARbuscule的发育和AM共生中的功能至关重要.
- 这些发现揭示了调节植物共生营养交换的新机制.
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