废除Lacticaseibacillus paracasei的腺甲基化能力,可以提高其冷干燥和储存耐受性
Hui Qiao1,2,3, Mingkun You1,2,3, Jiaming Yan1,2,3
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, People's Republic of China.
NPJ science of food
|May 19, 2025
概括
冷干燥初始培养可以降低生存能力. 一种非甲基化的Lacticaseibacillus paracasei突变体在冷干燥和储存后显示出更好的生存和活力,突出显示了腺甲基化在细菌稳定性中的作用.
科学领域:
- 食品微生物学 食品微生物学
- 工业生物技术 工业生物技术
- 分子生物学分子生物学
背景情况:
- 冷干燥对于初始培养保存至关重要,但往往会损害细胞活力.
- 细菌DNA甲基化,特别是腺甲基化,与细胞应激反应有关.
- 了解甲基化的作用可以提高工业微生物菌株的应用.
研究的目的:
- 调查腺因特异性DNA-甲基转移酶缺失对冷干燥后Lacticaseibacillus paracasei存活率的影响.
- 为了比较存储后非甲基化突变物与野生型菌株的活力和代谢概况.
- 阐明细菌腺因甲基化在初始培养稳定性中的作用.
主要方法:
- 使用了一种非甲基化的Lacticaseibacillus paracasei张突变缺乏腺因特异性DNA-甲基转移酶.
- 将突变型和野生型菌株进行冷干燥和30°C的储存,分别为30天和60天.
- 进行了转录组,蛋白组和代谢组分析,以评估代谢途径的差异.
主要成果:
- 与冷干燥和储存后的野生类型相比,非甲基化突变体显示出明显更高的细胞活力和存活率.
- 在存储的突变体中观察到明显的代谢途径改变,包括光转移酶系统,碳水化合物和氨基酸代谢以及脂肪酸生物合成.
- 这些代谢变化在转录基因,蛋白质基因和代谢基因数据集中得到了一致的识别.
结论:
- 缺少腺甲基化增强了Lacticaseibacillus paracasei对冷干燥和储存压力的弹性.
- 特定代谢途径的调节是非甲基化突变体稳定性的基础.
- 这项研究为优化工业初始培养提供了对细菌腺甲基化的新见解.
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