从制备到生物活性:对准备方法的比较研究和后生物质的表征
Amtalsaboor Almahbashi1, Evrim Gunes Altuntas1
1Ankara University Biotechnology Institute Ankara Turkey.
Food science & nutrition
|May 20, 2025
概括
后生物制备方法显著影响它们的生物活性. 无细胞超和热处理对于从乳酸细菌中产生后生菌是有效的,抗菌和抗氧化性质的菌株特异性变化.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 后生物质,来自细菌代谢的生物活性化合物,在食物中提供健康益处.
- 优化后生物生产需要了解制备方法和细菌菌株的影响.
研究的目的:
- 为了比较后生物制剂的无细胞超剂 (CFS),热和酶处理.
- 评估这些方法对乳酸细菌 (LAB) 活力,抗微生物活性和CFS生物活性的影响.
主要方法:
- 使用CFS,热处理和超声波酶处理制备后生物制剂.
- 使用的LAB菌株:乳球植物菌植物菌F2,Leuconostoc mesenteroides AF1和Pediococcus pentosaceus 50.这些菌株都在使用中.
- 评估了细胞活力,抗菌活性,抗氧化能力,总含量 (TPC) 和有机酸概况.
主要成果:
- 慢性疲劳和热处理有效地消除了可活的细菌细胞.
- 抗微生物活性取决于菌株和方法;CFS偏好L. plantarum F2和L. mesenteroides AF1,而热治疗偏好P. pentosaceus 50.
- L. plantarum F2表现出优越的抗氧化能力和TPC,与高乳酸和酸生产相关.
结论:
- 制备方法和细菌菌株选择极大地影响后生物产量和生物活性.
- 对于可扩展的后生物生产,CFS和热疗法是有希望的.
- 结果为优化后生物质用于食品保存应用提供了洞察力.
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