在酵母新陈代谢周期期间,对组织蛋白修饰的动态代谢调节
Bárbara Guzmán-Dinamarca1, Raúl Conejeros2, Marcelo Rivas-Astroza1
1Universidad Tecnológica Metropolitana, Departamento de Biotecnología, Santiago, Chile.
PloS one
|May 20, 2025
概括
代谢推动了酵母体的表观遗传变化,提供了像乙-甲酸和SAM这样的必不可少的构建块. 这些分子动态地影响着基因子修饰 (H3K9Ac,H3K4me3),影响酵母代谢周期中的基因表达.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 细胞表型通过表观遗传修饰得到稳定.
- 表观遗传多样化的起源仍然不清楚.
- 基因组后翻译修饰 (PTMs) 是关键的表观遗传标记.
研究的目的:
- 在Saccharomyces cerevisiae中研究表观基因辅基质生产和基因素PTM之间的相互作用.
- 了解在酵母代谢周期 (YMC) 中,乙-CoA和SAM流对H3K9Ac和H3K4me3的影响.
主要方法:
- 集成流量分析与转录基因数据.
- 分析了乙-CoA和SAM的生产流程.
- 检查的基因素标记是H3K9Ac和H3K4me3.3.
- 进行了基因本体学 (GO) 分析.
- 评估了染色体的可访问性.
主要成果:
- 在YMC期间,乙-CoA和SAM流动动态是异步的.
- 在代谢基因中,H3K9Ac丰富与乙-CoA相关.
- 在翻译相关基因中,H3K4me3丰富与SAM相关.
- 染色质可访问性是代谢流动驱动的基因组修饰的先决条件.
结论:
- 代谢途径提供及时的辅基质,这对基因素PTMs至关重要.
- 不同的代谢流量差异地调节特定的组织蛋白标记和相关的基因功能.
- 代谢和表观遗传学是动态相关的,推动表型多样化.
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