细胞组的加尔瓦诺塔克斯定向性取决于组的大小
Calina Copos1,2, Yao-Hui Sun3,4, Kan Zhu3,4
1Department of Biology, Northeastern University, Boston, MA 02115.
概括
细胞光作用,或电场中的定向迁移,取决于细胞群大小和PI3K抑制. 小小的PI3K受阻细胞群迁移到阳极,与较大的群体或不受阻碍的细胞不同.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 发育生物学 发展生物学
背景情况:
- 加尔瓦诺塔克斯是针对电场 (EF) 的定向细胞迁移,对伤口愈合和发育至关重要.
- 单个鱼类角质细胞在EF中以正极方式迁移,但PI3K抑制将其逆转为阳极方式.
- 大型细胞群,无论PI3K状态如何,都会以正极方式迁移,与单个细胞的行为形成鲜明对比.
研究的目的:
- 为了研究细胞群大小和PI3K抑制如何影响加尔瓦诺塔克斯.
- 阐明控制电场中集体细胞迁移的机制.
- 开发和验证一个解释观察到的群体迁移模式的计算模型.
主要方法:
- 在电场中对鱼类角质细胞细胞群迁移的实验观察.
- 药理上抑制PI3-激酶 (PI3K),以调节细胞行为.
- 在群体内细胞迁移动态的计算建模.
主要成果:
- 小小的未抑制的细胞群通过阴极迁移,而小小的PI3K抑制的细胞群通过阳极迁移.
- 在较小的群体和无抑制的群体中,与大小相似的PI3K受阻群体相比,迁移速度更高.
- 大群体的形状变化很小,而小群体的形状变形很大,在后边失去状体.
结论:
- 组内部和边缘的细胞以不同的方式解释定向EF信号.
- 内部细胞始终以阴极方向导向,边缘细胞则模仿单个细胞的行为.
- 群体光学方向是由内部和边缘细胞反应之间的平衡决定的,受PI3K状态和群体大小的影响.
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