在开花的白菜中吸收,细胞下积累和代谢14C-双S
Yandao Chen1, Yan Chen1, Sihan Yao1
1Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310058, China.
Journal of hazardous materials
|May 20, 2025
概括
双S (BPS) 是BPA的替代物,在开花的白菜中积聚,形成代谢物,就像食用叶子中的糖化形式一样. 这凸显了植物对BPS吸收和代谢的潜在食品安全风险.
科学领域:
- 环境科学 环境科学
- 植物生物学 植物生物学
- 毒理学 毒理学 毒理学
背景情况:
- 由于环境和健康问题,双S (BPS) 越来越多地被用作双A (BPA) 的替代品.
- 了解植物中的BPS吸收,代谢和积累对于评估对食品安全和人类健康的潜在风险至关重要.
- 水培培养提供了一个可控的环境来研究植物污染物相互作用.
研究的目的:
- 在水培条件下研究BPS在开花的白菜中的代谢途径和组织特异性积累.
- 为了识别BPS代谢物及其在植物内的亚细胞局部.
- 评估BPS在食用植物部位中的积累对食品安全的潜在影响.
主要方法:
- 花开的白菜在水培中暴露于14C标记的BPS (5毫克/升) 32天.
- 在营养溶液和植物组织 (根,茎,叶) 中量化14C放射性.
- 使用高性能液体染色学与液体闪光计数 (HPLC-LSC) 和液体染色学-质谱/质谱 (LC-MS/MS) 识别代谢物.
- 亚细胞分离以确定BPS及其代谢物的局部.
主要成果:
- 植物在32天内从营养溶液中吸收了60.2%的BPS.
- 在根 (40.2%),茎 (5.3%) 和叶子 (14.5%) 中检测到BPS及其代谢物,在老叶子中度更高.
- 确定了四种BPS代谢物,通过糖化,化,硫化和氨基酸结合形成.
- BPS和代谢物主要局限于细胞壁,塑和可溶性分片,导致根部的不可提取的残留物.
- 发现糖化代谢物,如M526,在可食用的植物部位中积聚.
结论:
- 开花的白菜通过各种结合途径代谢BPS,主要存储在细胞壁和塑性体等细胞区内.
- 在可食用组织中积累的BPS代谢产物,特别是糖化形式,构成了潜在的食品安全问题.
- 这些发现有助于了解植物排毒机制,并强调需要对农业系统中的BPS保持警.
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