相关实验视频

Updated: May 22, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

环境特有的光探针用于研究湖泊中氧化的检测机制

Zhe Chen¹, Ronghua Li¹, Syed Turab Raza²

¹State Key Laboratory for Vegetation Structure, Functions and Construction, Ministry of Education Key Laboratory for Transboundary Ecosecurity of Southwest China, and Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming 650500, China.

Journal of hazardous materials

|May 20, 2025

概括

研究人员开发了一种新的光探测器 (LR-P),用于快速监测湖泊中的氧化 (NO). 这种方法将光与NO水平和相关的微生物基因相关联,为湖泊循环提供了洞察力.

科学领域:

环境化学环境化学
微生物学微生物学
临界技术临界技术

背景情况:

氧化 (NO) 是一种重要的大气微量气体,影响空气质量和湖泊缩.
目前用于在水环境中检测NO的方法面临着诸如低分辨率和矩阵干扰等挑战.
在湖泊中需要快速,高分辨率和耐干扰的NO检测技术.

研究的目的:

引入和评估一个小分子光探针 (LR-P) 来检测氧化 (NO) 在湖泊中的分布.
建立一种可靠的水生生态系统中NO监测方法.
为了将NO检测与相关微生物功能基因的丰富性相关联.

主要方法:

使用小分子光探针 (LR-P) 通过光变化检测NO.
开发了一种多变量线性参数化,将光量子产量和强度与NO度相关联.
分析了与NO相关的微生物基因丰度 (amoA,nirS,nirK,nosZ) 和光数据之间的相关性.

主要成果:

在光量子产量和NO度 (R2=0.729,p<0.001) 之间建立了显著的相关性.
发现光量子产量与化和非化基因的丰富性之间存在显著的相关性.
证明了探测器在自然湖泊环境中检测NO和相关微生物丰度的能力.

结论:

该LR-P技术提供了一种快速,方便和防干扰的方法来分析湖泊中的NO分布.
这种基于光的方法可以作为评估NO生产微生物群落的代理.
将光技术与分子方法相结合,为NO检测和了解水生系统中循环提供了一个新的策略.

关键词:

脱基因的基因发生了变化.光探测器探测器可以发光.氧化氧化是什么?高原的湖泊高原的湖泊

更多相关视频

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...