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基于Tandem质量标签的蛋白质组学分析,以揭示Dendrobium nobile Lindl中的生长阶段依赖的途径

Wanhui Chen¹, Cong Xie², Wenying He¹

¹Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education and Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, Hainan 571158, China.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|May 20, 2025

概括

这项研究确定了Dendrobium nobile Lindl在不同生长阶段的283种不同表达的蛋白质. 老植物中高调节的抗氧化酶表明增强了应激弹性,有助于确定最佳收获时间.

科学领域:

蛋白质组学是指蛋白质组学.
植物生物学植物生物学
生物化学生物化学

背景情况:

丹德罗 (Dendrobium nobile Lindl) 是一种有价值的药用植物.
了解生长过程中的蛋白质表达变化对于优化种植和识别生物活性化合物至关重要.

研究的目的:

分析不同生长阶段在Dendrobium nobile Lindl中差异表达的蛋白质.
识别与植物生长和药理活性相关的功能蛋白质.

主要方法:

使用Tandem Mass Tag (TMT) 标签和LC-MS/MS的定量蛋白质组学.
对已识别的蛋白质进行生物信息分析.
对抗氧化酶活性 (SOD,POD,CAT) 的测定.

主要成果:

鉴定了6032种蛋白质,其中283种以不同的表达方式表达 (168种上调,115种下调).
蛋白质的显著变化与热冲击反应,烯胺生物合成和抗氧化活性有关.
在老植物中减少小热冲击蛋白 (sHSPs);在老植物中调节SOD,CAT和POD.

结论:

在生长阶段之间发现了283种具有不同功能的不同表达的蛋白质.
在较老的Dendrobium nobile Lindl中,高调节的抗氧化酶有助于氧化应激抵抗.
这些发现支持优化收获期和了解Dendrobium nobile Lindl.的药理机制.

关键词:

生物信息学分析 Dendrobium nobile Lindl 的生长阶段是什么?蛋白质组学是指蛋白质组学.双重质量标签 (TMT) 是一种

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