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Updated: May 22, 2025

Quantitative Analysis of Chromatin Proteomes in Disease
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用PerCell染色体测序测量差异性蛋白质基因组结合的高度定量测量

Alexi Tallan1, Jack Kucinski1, Benjamin Sunkel2

  • 1Nationwide Children's Hospital, Center for Childhood Cancer Research, Columbus, OH, USA; Molecular, Cellular, and Developmental Biology Program, The Ohio State University, Columbus, OH, USA.

Cell reports methods
|May 20, 2025
PubMed
概括

这项研究介绍了PerCell,一种使用细胞尖端进行精确的染色体测序数据定量比较的新方法. 珀塞尔能够进行可靠的跨物种表观遗传学分析,并促进数据标准化.

关键词:
科普:分子生物学CP: 系统生物学.下一个流程 下一个流程染色体序列测序 染色体序列测序跨物种的表观基因组学基因组学规范化规范化量化表观基因组学的研究.肉瘤 肉瘤是一种肉瘤.转录因子的转录因子

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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