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用至少2.5毫升/厘米无菌盐水洗,可以降低皮下组织模型中的细菌负载

Cody B Westmoreland¹, Sara D Lawhon², Jing Wu²

¹Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX.

American journal of veterinary research

|May 20, 2025

概括

至少2.5毫升/厘米的盐水洗有效降低了皮下组织模型中的细菌负载. 这项研究为手术伤口消毒提供了参考,指导了减少细菌的最佳洗量.

科学领域:

生物医学工程生物医学工程
微生物学微生物学
手术创新在外科创新.

背景情况:

手术部位感染 (SSIs) 是一个严重的问题,通常与细菌污染有关.
对手术伤口的有效消毒对于预防SSI至关重要.
确定最佳的灌量对于最大化细菌清除至关重要.

研究的目的:

在皮下组织模型中量化降低细菌负载到105 CFU/mL以下所需的洗量.
建立手术伤口洗有效性的基准.
为手术净化方案提供基于证据的指导.

主要方法:

基板实验研究使用20个无菌皮肤模型与10厘米的切口.
用Staphylococcus pseudintermedius或埃舍里希亚大肠杆菌注射大约1.5 X 108 CFU/mL.
在增量盐水冲洗量 (2.5,5和10毫升/厘米切口长度) 后的细菌量化.

主要成果:

所有测试的洗量都将细菌殖民地降低到S. pseudintermedius和E. coli的105 CFU/mL以下.
在2.5mL/cm洗时,观察到细菌负载的初始3-log降低.
进一步的洗量显示了增量减少,大肠杆菌从更高的体积中受益更多.

结论:

建议在线性手术伤口中至少进行2.5毫升/厘米的洗,以消毒皮下组织.
该模型为外科医生提供了一个参考,以确定有效的洗量.
需要进一步的临床研究来验证这些发现在活组织中.

关键词:

污染污染污染的污染是什么感染感染的感染.洗洗洗洗洗洗洗小动物动物小动物动物手术手术手术手术手术手术手术

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