从面包废物到细菌纤维素纳米结构:开发了一种新的旋转盘生物反应器
Sotirios Pilafidis1, Antiopi Vardaxi1, Konstantina Kourmentza2
1Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 11635 Athens, Greece.
International journal of biological macromolecules
|May 20, 2025
概括
一个新的生物反应器增强了细菌纤维素 (BC) 的生产,特别是使用面包废物. 经过修改的BC纳米结构显示了材料科学应用和可持续生物聚合物开发的潜力.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 环境可持续性 环境可持续性
背景情况:
- 细菌纤维素 (BC) 是一种多功能生物聚合物,具有众多应用.
- 需要有效和可持续的BC生产方法.
- 生物聚合物生产废物流的利用提供了环境和经济上的好处.
研究的目的:
- 设计和制造一种用于生产细菌纤维素 (BC) 的新型旋转盘生物反应器.
- 用Komagataeaeibacter rhaeticus优化BC生产,并探索废弃物价值化.
- 通过化学和酶来将BC转化为纳米结构 (BNC),并描述其特性.
主要方法:
- 开发一个具有优化参数的旋转磁盘生物反应器 (磁盘沉浸,旋转速度,空气供应).
- 使用商业葡萄糖和面包废弃物的酶化水解剂培养Komagataeaeibacter rhaeticus.
- 使用硫酸,盐酸和纤维素酶对BC进行现场修改,形成BNCs.
- 使用FTIR,动态光散射和传输电子显微镜 (TEM) 进行BC和BNC的表征.
主要成果:
- 最佳的生物反应器条件产生了1020毫克BC/盘与商业葡萄糖.
- 面包废弃物水解剂增加了133.3%的BC产量.
- 修改后的BNC表现出不同的纳米结构 (棒状,针状,带状),尺寸和宽度各不相同.
- FTIR证实了纤维素的存在,DLS显示了不同的尺寸分布.
结论:
- 新型旋转盘生物反应器促进了高效的细菌纤维素生产.
- 面包废弃物利用显著提高了BC产量,促进了可持续的生物聚合物制造.
- 量身定制的BC纳米结构产生了具有可调节性质的材料,用于先进的应用.
- 这种综合方法通过废物利用和创新的生物反应器设计,促进生物技术和环境可持续性.
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