相关实验视频

Updated: Jun 14, 2025

Author Spotlight: Isolation of Long Muscle Fibers from Mouse Hindlimb Muscles for Studying Excitation-Contraction Coupling Across Fiber Types

Author Spotlight: Isolation of Long Muscle Fibers from Mouse Hindlimb Muscles for Studying Excitation-Contraction Coupling Across Fiber Types

Published on: December 1, 2023

综合单细胞多基因分析显示,肌肉纤维类型的基因调节电路是由耐力运动调节的

Aliza B Rubenstein¹, Gregory R Smith¹, Zidong Zhang^1,2

¹Department of Neurology, Icahn School of Medicine at Mount Sinai (ISMMS), New York, New York 10029, USA.

Genome research

|May 20, 2025

概括

急性耐力运动会在特定类型的骨肌肉细胞中触发独特的基因调节反应. 这项研究绘制了这些细胞特异性的分子变化,揭示了介导运动的关键电路.

科学领域:

* 运动生理学
* 分子生物学 * 分子生物学
* 基因组学是一个学科.

背景情况:

*骨肌肉适应耐力运动对于表现和健康至关重要.
*基因调节回路是运动效应的关键媒介,但在肌肉中的单细胞水平上仍未研究.
*以前的研究通常依赖于大量组织分析,掩盖细胞类型特定的反应.

研究的目的:

* 为了全面地绘制基因调节电路对个人骨肌肉细胞类型中的急性耐力运动的反应.
* 通过运动调节的共享和细胞类型特定的分子程序.
* 创建一个有价值的资源,以了解运动的分子基础.

主要方法:

*使用同细胞RNA-seq/ATAC-seq多基因测定在巨大的横向肌肉样本上.
*分析了来自37154个细胞核的14种细胞类型的数据,在运动或休息前和之后进行循环运动.
* 采用单细胞调节电路三元重建.

主要成果:

* 识别了不同肌肉细胞类型和纤维类型 (缓慢,快速,中等) 的独特基因表达和染色质可访问性变化.
* 在表达光子 (LUM) 的纤维-基原始细胞中发现了显著的调控事件.
*重建了运动调节的基因调节电路,涉及328个转录因子和2025个基因.

结论:

*急性耐力运动会在不同的骨肌肉细胞群中诱导高度特定的基因调节电路反应.
*这项研究提供了运动诱导的肌肉分子变化的详细单细胞地图.
* 本资源有助于理解运动生理学和解释大量组织数据.

更多相关视频

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Published on: September 8, 2023

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

相关概念视频

Exercise and Muscle Performance

Exercise and Muscle Performance

Exercise induces a range of adaptations in muscle tissue, depending on the type and duration of activity. Such physical training can be broadly categorized into two types: endurance exercises and resistance exercises.
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...

Classification of Skeletal Muscle Fibers

Classification of Skeletal Muscle Fibers

Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...