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透露TCA循环流动力学在杀剂适应的Botrytis cinerea通过Anionic染色学同时分离

Yanjun Zhao¹, Sijia Zhao², Xiaowei Wang²

¹Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.

Journal of agricultural and food chemistry

|May 20, 2025

概括

这项研究通过分析TCA循环中间体,使用阴离子染色学来监测Botrytis cinerea真菌杀菌剂的耐药性. 杀菌剂的作用有所变化,揭示了博特里斯病毒管理的新策略.

科学领域:

农业科学农业科学
生物化学生物化学
菌类学菌类学是指菌类学.

背景情况:

有效监测 * Botrytis cinerea * 种群对于管理这种重要的植物病原体至关重要.
了解杀真菌剂的作用机制是调整疾病控制策略的关键.

研究的目的:

开发一种方法,同时分离和量化所有三碳酸 (TCA) 循环中间体在B. cinerea*.
调查三种杀菌剂pyraclostrobin (PYR),tebuconazole (TEB) 和carbendazim (CAR) 对TCA循环代谢的影响.
评估TCA循环分析对监测杀菌剂耐药性和指导疾病管理的潜力.

主要方法:

动态色谱被用于有效和同时分离九种有机酸和六种无机离子,包括所有TCA循环中间体.
用不同度的PYR,TEB和CAR.处理了*B. cinerea*的菌培养.
在治疗后48小时和96小时分析了代谢物水平,以评估TCA循环中间体的变化.

主要成果:

所有测试的杀菌剂都以度依赖的方式抑制了菌根的生长,尽管长时间暴露减少了抑制.
TEB和PYR对TCA循环中间体表现出明显的影响,TEB改变了烟酸盐,酸盐,酸盐,异酸盐,酸盐和α-酸盐的水平,而PYR对烟酸盐,酸盐,酸盐和酸盐表现出双相影响.
CAR显著减弱了TCA循环流,导致异酸盐和α-酸盐的持续下降,并对氧酸盐积累产生了差异影响.
在TCA循环中间体中,杀剂诱导的变化在治疗之间和随着时间的推移而有显著差异.

结论:

阴离子色谱提供了一种精确的方法来评估B. cinerea*中的TCA循环功能.
对TCA循环中间体的代谢分析可以揭示对杀菌剂的差异反应,从而提供对抗性机制的见解.
这些发现支持制定有针对性的杀真菌策略,并改善对B. cinerea*种群的监测,以实现可持续的疾病管理.

关键词:

这种植物是Botrytis cinerea.在TCA循环流量流程中,杀菌剂适应的适应方法同时分离的同时分离

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