相关实验视频

Updated: May 23, 2025

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

对基因编辑代谢干扰和免疫疗法的反应性氧物种响应纳米电机

Zhiyong Liu¹, Xiaowei Luan¹, Qianglan Lu¹

¹Department of Gastric and Hernia Surgery, Nanjing Drum Tower Hospital, College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210006, Nanjing, China.

Nature communications

|May 20, 2025

概括

这项研究引入了一种用于癌症治疗的新型纳米电机,该电机使用活性氧物种 (ROS) 向瘤. 纳米电机增强基因编辑和免疫反应,显著抑制固体瘤的生长.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
在瘤学瘤学.

背景情况:

传统的基因编辑载体与固体瘤透斗争.
针对瘤代谢和免疫逃避对于有效的癌症治疗至关重要.

研究的目的:

开发一种ROS驱动的纳米电机,用于增强瘤透和多层次的癌症干预.
研究纳米电机在抑制固体瘤生长和调节瘤微环境方面的有效性.

主要方法:

开发一个半核纳米电机,封装CRISPR/Cas9等离子体,准LDHA (RDN@PL).
利用细胞外ROS进行纳米运动自我扩散和内积累.
采用血红素氧酶-1 (HO-1) 进行纳米运动降解,CO释放和等离子体输送.
评估瘤生长抑制,代谢变化,免疫细胞透和亡诱导.

主要成果:

与被动颗粒相比,RDN@PL显示出更高的内积累.
LDHA淘汰和CO释放抑制了葡萄糖分解和增加了线粒体ROS,诱导了亡.
通过纳米电机清理细胞外ROS,逆转了免疫细胞死亡抑制,增强了CD8+ T细胞的透.
在固体瘤模型中达到93.9%的瘤生长抑制.

结论:

由ROS驱动的纳米电机为克服固体瘤透障碍提供了一个有希望的策略.
这种多层次的干预方法有效地结合了基因编辑,代谢干扰和免疫增强,用于癌症治疗.
工程化纳米微粒为精密瘤学应用提供了一个蓝图.

更多相关视频

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Published on: June 14, 2018

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...