DNA甲基化通过影响太平洋白 (Litopenaeus vannamei) 的代谢途径来调节生长特征
Xin Zhang1, Chaoqun Hu1, Ting Chen1
1Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (KLBBSA), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
BMC genomics
|May 20, 2025
概括
DNA甲基化与太平洋白 (Litopenaeus vannamei) 的生长有负相关性. 缓慢生长的中较低的DNA甲基化对代谢基因进行了上调,这表明改变了活性,而不是增长分配.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 水产养殖是水产养殖的一种方式.
- 基因组学就是基因组学.
背景情况:
- 太平洋白 (Litopenaeus vannamei) 是一个关键的水产养殖物种,其主要经济特征是生长.
- 了解DNA甲基化在调节生长中的作用至关重要,但仍未得到充分研究.
- 这项研究调查了与L. vannamei.生长性能相关的DNA甲基组动态.
研究的目的:
- 确定与L. vannamei.不一致的生长率相关的DNA甲基化模式.
- 探索将DNA甲基化与生长特征联系起来的调控机制.
- 分析生长中的DNA甲基化和基因表达之间的相互作用.
主要方法:
- 全基因组双硫酸盐测序 (WGBS) 用于分析DNA甲基组.
- 转录组分析以评估基因表达.
- 甲基化和表达数据的综合分析.
主要成果:
- 缓慢生长的 (SG) 的DNA甲基化基因表达高于快速生长的 (FG) .
- 与FG个体相比,WGBS显示SG个体的肌肉甲基化水平下降.
- 鉴定了532个差异甲基化促进体和2067个差异甲基化区域,其中47个重叠的差异甲基化和表达基因 (DMGs/DEGs) 富含在代谢途径中.
结论:
- 基因甲基化是L. vannamei生长特征的关键调节者,与生长有负相关性.
- 基因甲基化通过上调代谢途径基因来影响生长,特别是在葡萄糖和氨基酸代谢中.
- 较高的代谢活性,由改变的DNA甲基化表明,可能是缓慢生长的的应激反应,从生长转移资源.
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